Recovery of Lysozyme from Hen Egg White by means of Selective Magnetic Cake Filtration
- Autor: Eichholz, C. / Silvestre, M. / Franzreb, M. / Nirschl, H. (2011)
Engineering in Life Sciences 11 (2011), 1, 75–83
- Datum: 2011
Eichholz, C. / Silvestre, M. / Franzreb, M. / Nirschl, H. (2011): „Recovery of Lysozyme from Hen Egg White by means of Selective Magnetic Cake Filtration“. In: Engineering in Life Sciences 11 (2011), 1, 75–83
A new concept for the improvement of the downstream processing and purification is the so-called magnetic separation. By using surface functionalized magnetic substrate particles, which selectively adsorb the target product, it can be directly separated out of a crude bioprocess stream. These methods are already used for analytical purposes, where only small amounts of functionalized particles are necessary.
To apply the same concept at a larger scale, effective and economical procedures have to be provided. First, suitable process equipment has to be developed. Second, the magnetic particles have to be manufactured with a stable surface functionalization and long-term stability for their reuse. Up to now mainly high-gradient magnetic separation filter devices are applied for selective magnetic separation.They consist of a magnetic matrix in which the magnetic particles are trapped.
In this work, a new magnetic filter is introduced that overcomes the capacity limitations of the current high-gradient magnetic separation technology. The principle is demonstrated by selective recovery of lysozyme from hen egg white. Prior to the separation experiments magnetic beads with a strong acid cation-exchange surface functionalization are synthesized. The separation procedure is implemented in only one unit operation. With the implementation of the displacement elution sequence lysozyme can be separated out of a hen egg white solution with a purification factor of PF536 and a purity P50.83.