Knock‐out of multidrug efflux pump MexXY‐OprM results in increased susceptibility to antimicrobial peptides in Pseudomonas aeruginosa

  • chair:

    Neidig, A. / Strempel, N. / Waeber, N. B. / Nizer, W. S. d. C. / Overhage, J. (2023)

  • place:

    Microbiology and Immunology 2023, 67, 9, 422-427

  • Date: Juli 2023
  • Abstract

    Multidrug efflux systems of the resistance-nodulation-cell division family play a crucial role in resistance of Pseudomonas aeruginosa to a large variety of antibiotics. Here, we investigated the role of clinically relevant efflux pumps MexAB−OprM, MexCD−OprJ, and MexXY−OprM in resistance against different cationic antimicrobial peptides (AMPs). Our results indicate that a knock-out in efflux pump MexXY-OprM increased susceptibility to some AMPs by two- to eightfold. Our data suggest a contribution of MexXY-OprM in resistance to certain AMPs in P. aeruginosa, which should be considered in the future development of new and highly active antimicrobial peptides to fight multidrug resistant infections.