Biofunctionalization of Metal–Organic Framework Nanoparticles via Combined Nitroxide‐Mediated Polymerization and Nitroxide Exchange Reaction

  • chair:

    Wagner, I. / Spiegel, S. / Brückel, J. / Schwotzer, M. / Welle, A. / Stenzel, M. H. / Bräse, S. / Begum, S. / Tsotsalas, M. (2023)

  • place:

    Macromolcular Materials and Engineering, 2023, 308, 9

  • Date: Mai 2023
  • Abstract

    Surface engineering of metal–organic framework nanoparticles (MOF NPs), and enabling their post-synthetic modulation that facilitates the formation of bio-interfaces has tremendous potential for diverse applications including therapeutics, imaging, biosensing, and drug-delivery systems. Despite the progress in MOF NPs synthesis, colloidal stability and homogeneous dispersity—a desirable property for biotechnological applications, stands as a critical obstacle and remains a challenging task. In this report, dynamic surfaces modification of MOF NPs with polyethylene glycol (PEG) polymer is described using grafting-from PEGylation by employing nitroxide-mediated polymerization (NMP) and inserting arginylglycylaspartic acid (RGD) peptides on the surface via a nitroxide exchange reaction (NER). The dynamic modification strategy enables tailoring PEG-grafted MOF NPs of the type UiO-66-NH2 with improved colloidal stability, and high dispersity, while the morphology and lattice crystallinity are strictly preserved. The interaction of PEG-grafted MOF NPs with human serum albumin (HSA) protein under physiological conditions is studied. The PEG-grafted colloidal MOF NPs adsorb less HSA protein than the uncoated ones. Therefore, the described approach increases the scope of bio-relevant applications of colloidal MOF NPs by reducing nonspecific interactions using NMP based PEGylation, while preserving the possibility to introduce targeting moieties via NER for specific interactions.