Institute of Functional Interfaces

Indirect thermal lens detection for capillary electrophoresis

  • chair:

    Nedosekin, D.A. / Bendrysheva, S.N. / Faubel, W. / Proskurnin, M.A. / Pyell, U. (2007)

  • place: Talanta 71 (2007), 1788-1794

  • Date: 2007
  • Nedosekin, D.A. / Bendrysheva, S.N. / Faubel, W. / Proskurnin, M.A. / Pyell, U. (2007): „Indirect thermal lens detection for capillary electrophoresis“. In: Talanta 71 (2007), 1788-1794

Abstract

Thermal lens detection with a 325.0 nm He–Cd excitation laser is used for thermooptical indirect detection in combination with the capillary electrophoretic separation of organic anions. The optimization of indirect thermooptical detection is discussed. With Mordant Yellow 7 (an azo dye) chosen as a probe ion limits of detection for 1-heptane-, 1-pentane-, 1-butane-, 1-propanesulfonic, and acetic acid at a level of n × 10−7 M were achieved with a separation electrolyte containing 50 μM of the probe ion and 5 mM Tris pH 9.90. A further increase in the detection sensitivity (twofold decrease in the limit of detection ) was obtained with a separation electrolyte containing a volume fraction of 20% acetonitrile.


 

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