Optimization of enzyme immobilization on magnetic microparticles using 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDC) as a crosslinking agent
F. Kazenwadel, F. / Wagner, H. / B. E. Rapp, B:, E. / Franzreb, M. (2015)
Analytical Methods, 2015, 7, 10291-10298
- Date: November 2015
For GOx the optimization of the immobilization parameters pH-value and the enzyme to particle ratio results in activity yields of up to 36%, which is in the usual range for undirected enzyme immobilisations. In contrast, for HRP the activity yield does not exceed 6% even after optimization of the protocols.