Recombinant hydrophobin coated surfaces and their influence on microbial biofilm formation
Rieder, A. / Ladnorg, T. / Wöll, C. / Obst, U. / Fischer, R. / Schwartz, T. (2011)
VAAM 2011, Karlsruhe, Deutschland, 3.-6. April, 2011
- Date: 2011
Rieder, A. / Ladnorg, T. / Wöll, C. / Obst, U. / Fischer, R. / Schwartz, T. (2011): „Recombinant hydrophobin coated surfaces and their influence on microbial biofilm formation“. In: VAAM 2011, Karlsruhe, Deutschland, 3.-6. April, 2011
Thus, protocols for surface coating with recombinant fusion hydrophobins were developed. Quartz crystal microbalance measurements were used to analyze the adsorption behaviour of the fusion hydrophobins. The hydrophobin coatings were characterized with water contact angle measurements, immunofluorescence microscopy and atomic force microscopy in terms of hydrophobicity and homogeneity. The self-assembly process of the recombinant fusion hydrophobins depended on the incubation temperature and the incubation time. Fusion hydrophobins are as well suited as natural hydrophobins for surface modification.
To test the possible application of hydrophobins for antifouling coatings, the growth behaviour of various microorganisms was studied on hydrophobin modified versus unmodified glass surfaces. Single bacterial strains as well as natural bacterial communities were used to analyse biofilm formation. Apart from conventional plating experiments, fluorescence microscopy and molecular-biological methods such as denaturing gradient gel electrophoresis were applied to determine differences in the biofilm growth. The results demonstrated that the change of surface hydrophobicity and the fusion hydrophobins itself did not affect the biofilm formation.
Due to their self-assembly properties, fusion hydrophobins can be used for effective large-scale surface coating in monolayer manner. To stimulate the effect on biofilm formation the hydrophobins can subsequently be functionalized with already bioactive molecules like antimicrobial peptides to influence the bacterial adhesion.