Institute of Functional Interfaces

Bachelor or Masterthesis

Master thesis (from 06/2020)

„Development of an in vitro test system for 3rd generation CAR-T-cells“

Cellular immune therapies are gaining more and more attention. Among these are those using recombinant T-cell receptors or chimeric antigen receptors (CAR-T-cells). These tumor specific T-cells display a huge potential that has already been shown in various tumor species. The broader application of these new therapies is hampered by the high costs and the lack of appropriate in vitro test systems. Therefore, the master thesis topic will be dedicated to the development of a microcavity array-based in vitro system which will be able to determine the potency of CAR-T-cells prior to infusion into the patient. For this, a leukemic stem cell niche will be developed that allows for the longitudinal observation of CAR-T-cells in a microbioreactor system (4D-microscopy).

The thesis will be conducted in the framework of a cooperation project with the University Clinic in Heidelberg and offers the possibility of using primary material. The results of the thesis will be highly valuable since they will have a direct impact on the use of CAR-T-cells within a running CAR-T-cell study.

If you are interested in micro systems, stem cells and confocal microscopy or should you need more information, please contact:

Prof. Dr. Eric Gottwald

Email: eric gottwaldTsy3∂kit edu

Phone: 0721/608-26803

 

 

 

Bachelor/Master thesis (immediate beginning)

„Characterization of hepatocytes in microcavity arrays modified with collagen fibres produced by electro-jetting“

Microcavity arrays have been proven useful as a versatile 3D cell culture platform. Besides their application as artificial stem cell niches for hematopoietic and leukemic stem cells, they have long been known as a platform for the organotypic culture of hepatocytes. Typically, the cells inside microcavities synthesize their own extracellular matrix, especially during long term experiments, so that no additional extracellular matrix components have to be added. In the planned project, the organotypic performance of hepatocytes cultured in collagen fiber scaffolds inside microcavities will be characterized. The so-called electro-jetting process is tunable with regard to crosslinking and fiber diameter, therefore it will be of interest whether the behavior of hepatocytes in modified microcavities will be different form unmodified ones.

If you are interested in micro systems, confocal microscopy and metabolic assays or should you need more information, please contact:

Prof. Dr. Eric Gottwald

Email: eric gottwaldMoe2∂kit edu

Phone: 0721/608-26803

 

 

Bachelor/Master thesis (immediate beginning)

„Development of cell printing strategies for the automated inoculation of  microcavity arrays“

Microcavity arrays have been proven useful as a versatile 3D cell culture platform. Besides their application as artificial stem cell niches for hematopoietic and leukemic stem cells, they have long been known as a platform for the organotypic culture of hepatocytes.

In the planned project, 3D printing strategies for the automated inoculation of various cell types into microcavity arrays shall be developed. For this, the available 3D-printing techniques will be evaluated for their suitability to print into 300 µm small microcavities. After the development of suitable printing protocols, the cells will be cultivated for several days und characterized with regard to their physiological properties.

If you are interested in 3D cell printers, micro systems, confocal microscopy and metabolic assays or should you need more information, please contact:

Prof. Dr. Eric Gottwald

Email: eric gottwaldWju7∂kit edu

Phone: 0721/608-26803

 

 

Topics for theses are always continuously assigned

 

Start: immediately or from 06/2020
Contact: Prof. Dr. Eric Gottwald
Institute of Functional Surfaces, AG 3D-Cellculture Systems
Eric gottwaldLqv9∂kit edu