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Isomaltose formation by free and immobilised dextransucrase

Isomaltose formation by free and immobilised dextransucrase
chair:

Berensmeier, S. / Jördening, H.-J. / Buchholz, K. (2006)

place:

Biocatalysis and Biotransformation 24 (2006), 4, 280–290

Date: 2006

Berensmeier, S. / Jördening, H. / Buchholz, K. (2006): „Isomaltose formation by free and immobilised dextransucrase“ In: Biocatalysis and Biotransformation 24 (2006), 4, 280–290

Abstract

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The acceptor reaction of dextransucrase from Leuconostoc mesenteroides NRRL-B512F with glucose as acceptor is of technical interest for isomaltooligosaccharide (IMOs) synthesis. Different experimental conditions were investigated for free and immobilized enzyme. The data for oligosaccharide formation up to a degree of polymerization 4 were correlated with a model developed earlier, and optimal reaction conditions for immobilized dextransucrase design and application were identified for later continuous application. Furthermore, stability was investigated for free and immobilized enzyme including stabilization by sugars.