Institute of Functional Interfaces

Video/ Jonas Huebner 2016

Methods

 

Microbiological methods

 

  • Cultivation, isolation of microorganisms on selective nutrient media

     

  • Specific cultivation systems of indigenous bacterial populations from natural clay raw materials


 

Molecular biological analysis of bacterial populations

 

  • Extraction of specific microbial DNA from clay raw materials, minerals and surface materials

     

  • DNA-Fingerprint analysis of 16S-rDNA (PCR-DGGE)

     

  • Realtime-PCR (Taqman-PCR)

     

  • Sequence analysis of specific gens and identification of microorganisms

     

 

 

Imaging methods

 

 

  • AFM, atomic-force microscopy

     

  • BioAFM, simultanous detection of surface structure and fluorescence microscopy

     

  • Fluorescence-in-situ-hybridisation (FISH)

     

  • ESEM (environmental-scanning-electron-microscopy )

 

 

Metabolic activities

 

  • Enzyme activities: Detection of enzymatic metabolic activities in microbial populations by chromogenic and fluorogenic substrates

 

 

Mineralogic analysis

 

  • STA

     

  • XRD

     

  • CEC