Holographic microscopy

Tracking_EN



Many colonization assays analyze snapshots in time. As complete mechanistic understanding of the kinetics of surface colonization requires knowledge about the settlement behavior of individual microorganisms, we develop 3D tracking techniques, such as digital in-line holography and stereoscopy which allow tracking swimming microorganisms in four dimensions (three spatial and one temporal dimension).

With the obtained trajectories exploration patterns can be analyzed and coating induced motility changes are quantified. Analysis of the velocity- and angular distributions allow determining interaction distances. We also apply 3D tracking methods in life and medical sciences (e.g. motility of pathogenic trypanosomes, bacterial motility).

 

 

The work is supported by

 

ONR

ONR (Office of Naval Research)

 

 

 

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DFG

HoloVis(Deutsche Forschungsgemeinschaft)

 

 

 

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SEACOAT

SEACOAT (Surface Engineering for Antifouling - Coordinated Advanced Training)

 

 

 

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BioInterfaces

BioInterfaces Program

 

 

 

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Selected articles/Holographic microscopy
Title Author Source
Digital In-line Holography as three dimensional tool to study motile marine organisms during their exploration of surfaces

Heydt, M. / Rosenhahn, A. / Grunze, M. / Pettitt, M. / Callow, M. / Callow, J. (2007)

The Journal of Adhesion 83 (2007), 417
Analysis of holographic microscopy data to quantitatively investigate three dimensional settlement dynamics of algal zoospores in the vicinity of surfaces

Heydt, M. / Divos, P. / Grunze, M. / Rosenhahn, A. (2009)

European Physics Journal E 30 (2009), 141
Classification of Swimming Microorganisms Motion Patterns in 4D Digital In-Line Holography Data

Leal-Taixe, L. / Heydt, M. / Weisse, S. / Rosenhahn, A. / Rosenhahn, B. (2010)

DAGM 2010 - 32nd Annual Symposium for Pattern Recognition, Darmstadt, Deutschland, 22.-24 September, 2010
A stereoscopic approach for three dimensional tracking of marine biofouling microorganisms

Maleschlijski, S. / Leal-Taix´e, L. / Weiße, S. / Di Fino, A. / Aldred, N. / Clare, A. / Sendra, G. / Rosenhahn, B. / Rosenhahn, A. (2011)

MIAAB (2011)
Flow conditions in the vicinity of microstructured interfaces studied by holography and implications for the assembly of artificial actin networks

Weiße, S. / Heydt, M. / Maier, T. / Schulz, S. / Spatz, J. / Grunze, M. / Haraszti, T. / Rosenhahn, A. (2011)

Phys. Chem. Chem. Phys. 13 (2011), 29, 13395