Complex ciprofloxacin resistome revealed by screening a Pseudomonas aeruginosa mutant library for altered susceptibility

  • chair:

    Breidenstein, E. / Khaira, B. / Wiegand, I. / Overhage, J. / Hancock, R. (2008)
  • place:

    Antimicrobial Agents and Chemotherapy 52 (2008), 12, 4486-4491
  • Date: 2008

  • Breidenstein, E. / Khaira, B. / Wiegand, I. / Overhage, J. / Hancock, R. (2008): „Complex ciprofloxacin resistome revealed by screening a Pseudomonas aeruginosa mutant library for altered susceptibility“. In: Antimicrobial Agents and Chemotherapy 52 (2008), 12, 4486-4491

Abstract

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Pseudomonas aeruginosa offers substantial therapeutic challenges due to its high intrinsic resistance to many antibiotics and its propensity to develop mutational and/or adaptive resistance. The PA14 comprehensive mutant library was screened for mutants exhibiting either two- to eightfold increased susceptibilities (revealing genes involved in intrinsic resistance) or decreased susceptibilities (mutational resistance) to the fluoroquinolone ciprofloxacin. Thirty-five and 79 mutants with increased and decreased susceptibilities, respectively, were identified, as confirmed by broth dilution.