Hänsch, G.M. / Brenner-Weiss, G. / Prior, B. / Wagner, C. / Obst, U. (2008): „The extracellular substance of Pseudomonas aeruginosa: too slippery for neutrophils to migrate on?“. In: Int. J. Artif. Organs. 31 (2008), 9, 796-803
The extracellular substance of Pseudomonas aeruginosa: too slippery for neutrophils to migrate on?
Hänsch, G.M. / Brenner-Weiss, G. / Prior, B. / Wagner, C. / Obst, U. (2008)
|place:||Int. J. Artif. Organs. 31 (2008), 9, 796-803|
Biofilm formation is increasingly recognized as the cause of persistent infections and there is evidence that P. aeruginosa organized into biofilms are quite resistant toward host defence mechanisms, particularly against an attack by polymorphonuclear neutrophils (PMN). Apparently, the migration of PMN through the biofilms is impaired, and thus the bactericidal activity remains highly localized. The aim of this study was to directly investigate the interaction of PMN with the biofilm and the extracted extracellular polymeric substance (EPS) of P. aeruginosa.
Chemotaxis and random migration of PMN through P. aeruginosa biofilms was tested, as was their migration through and along the EPS. We found that the EPS and mature biofilms, but not immature or developing ones, reduced the chemotactic migration of PMN. On EPS, rather than immobilize the cells, their random, spontaneous migration was enhanced. We propose that on EPS, the PMN lose their capacity to sense the direction and just slide over the EPS in a disoriented manner.