Potential controlled DNA- Separation
DNA- Separation and purification from cell lysates is one of the main goals in modern biotechnology. Current techniques are chemically controlled and require a variety of buffer solutions. In order to reduce this need for buffer solutions, a new approach
considers the process of potential-controlled DNA separation onto porous carbon electrodes. This procedure is based on the principle of the so-called capacitive deionisation, which is currently mainly used for the desalination of wastewater. The idea behind the process is to polarize a conductive material and adsorb ions of opposite charge. Due to the applied potential, this principle is therefore called electrosorption. As carbon materials show high conductivity values and high specific surface areas they prove to promising for this application. Currently a high number of carbon materials is available. Therefore, this project focuses on the characterization of different morphologies in order to determine material parameters such as pore sizes and pore size istributions, which are important for the process of potential-controlled DNA purification. Furthermore, the influence of electrical properties of the materials, such as the conductivity, have to be investigated. The aim of the project is to determine material properties with the aid of different experimental and simulative methods that enable an optimal yield of adsorbed molecules. An evaluation of the sorption properties can be determined on the basis of calculating the specific adsorption capacity.
Figure 1: Experimental test setup and principle of a capacitive deionisation process (CDI)